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rna sequencing transcriptome library  (Illumina Inc)


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    Structured Review

    Illumina Inc rna sequencing transcriptome library
    a Schematic illustration of the <t>RNA-seq</t> and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.
    Rna Sequencing Transcriptome Library, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 12811 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptome+libraries/TruSeq+RNA+Library+Prep/pmc12946169-402-1-16
    Average 99 stars, based on 12811 article reviews
    rna sequencing transcriptome library - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "A DNA-based nanodevice senses purinergic signaling and drives an immune switch for resolving inflammation"

    Article Title: A DNA-based nanodevice senses purinergic signaling and drives an immune switch for resolving inflammation

    Journal: Nature Communications

    doi: 10.1038/s41467-026-68879-2

    a Schematic illustration of the RNA-seq and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.
    Figure Legend Snippet: a Schematic illustration of the RNA-seq and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.

    Techniques Used: RNA Sequencing, Western Blot, Comparison

    Related Articles

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    Article Title: Gene mapping and candidate gene analysis of a sorghum sheathed panicle‐I mutant
    Article Snippet: Three biological replicates were established for each developmental stage, and total RNA was extracted from the different tissues at each time point using TRIzol Reagent (Invitrogen). .. Transcriptome libraries were constructed utilizing the TruSeq RNA Library Prep Kit (Illumina) according to the manufacturer's protocol, followed by high‐throughput sequencing conducted on the Illumina NovaSeq 6000 platform. ..

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    Article Snippet: RNA integrity was assessed using the RNA 6000 Nano Kit with the Agilent 2100 BioAnalyzer (Agilent Technologies, CA, USA). .. Subsequently, transcriptome libraries were constructed and sequenced on the Illumina NovaSeq 6000 platform. ..

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    Article Title: Unveiling detoxifying symbiosis and dietary influence on the Southern green shield bug microbiota
    Article Snippet: Prior to sequencing, bacterial mRNA was purified with the MICROBExpress Bacterial mRNA Enrichment Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Transcriptome libraries were constructed using the TruSeq Stranded mRNA Library Prep protocol (Illumina) according to the manufacturer’s instructions. ..

    Next-Generation Sequencing:

    Article Title: Gene mapping and candidate gene analysis of a sorghum sheathed panicle‐I mutant
    Article Snippet: Three biological replicates were established for each developmental stage, and total RNA was extracted from the different tissues at each time point using TRIzol Reagent (Invitrogen). .. Transcriptome libraries were constructed utilizing the TruSeq RNA Library Prep Kit (Illumina) according to the manufacturer's protocol, followed by high‐throughput sequencing conducted on the Illumina NovaSeq 6000 platform. ..

    Sample Prep:

    Article Title: Molecular insights into dye decolorization performance and mechanisms under carbon limited conditions in a membrane aeration-based bioelectrochemical system.
    Article Snippet: Bioelectrochemical systems (BES) is a promising strategy for azo dyes decolorization enhancement in carbonlimited condition, but decolorization products need further aerobic mineralization.. Here, a counter-diffusion biofilm-supported BES (E-MABR) was designed to achieve the decolorization, mineralization and denitrogenation of Alizarin Yellow R (AYR) in electron-deficient wastewater.. The introduction of electrodes facilitated the secretion of extracellular polymeric substances (EPS), particularly proteins (PN), whose content in the cathodic biofilm was 2.5 ± 0.2 times higher than that in the MABR.

    Article Title: Unravelling the postharvest quality diversities of different sweet corn varieties
    Article Snippet: Different varieties of sweet corn exhibit varying degrees of quality deterioration after harvest, especially changes in appearance, sugar composition, texture and flavor.. Changes of 18 common phenolic acids, organic acids, and sugars of sweet corn varieties, ‘Jingkenuo 2000’, ‘Jingkenuo 768’ and ‘Nongkenuo 336’, during a shelf-life period of 10 d at 20 C were identified in this study.. The differences of flavor among the varieties may be due to their different flavor regulation pathways.

    RNA Extraction:

    Article Title: Unravelling the postharvest quality diversities of different sweet corn varieties
    Article Snippet: Different varieties of sweet corn exhibit varying degrees of quality deterioration after harvest, especially changes in appearance, sugar composition, texture and flavor.. Changes of 18 common phenolic acids, organic acids, and sugars of sweet corn varieties, ‘Jingkenuo 2000’, ‘Jingkenuo 768’ and ‘Nongkenuo 336’, during a shelf-life period of 10 d at 20 C were identified in this study.. The differences of flavor among the varieties may be due to their different flavor regulation pathways.

    Selection:

    Article Title: Chromosome-level genome assembly of Niphotrichum japonicum provides new insights into heat stress responses in mosses
    Article Snippet: DNA libraries for short-read whole genome sequencing (WGS) were constructed using the Illumina TruSeq DNA PCR-free library preparation kit (Illumina, CA, USA) with 300- to 500-bp fragment sizes, and sequenced on the Illumina NovaSeq 6000 platform to generate 150-bp paired-end (PE) reads. .. Transcriptome libraries were constructed with a TruSeq RNA Library Prep Kit v2 (Illumina, CA, USA) with an insert size of 200–400 bp, after polyA selection, and sequenced on an Illumina NovaSeq 6000 platform, and 150-bp PE reads were generated. ..

    Generated:

    Article Title: Chromosome-level genome assembly of Niphotrichum japonicum provides new insights into heat stress responses in mosses
    Article Snippet: DNA libraries for short-read whole genome sequencing (WGS) were constructed using the Illumina TruSeq DNA PCR-free library preparation kit (Illumina, CA, USA) with 300- to 500-bp fragment sizes, and sequenced on the Illumina NovaSeq 6000 platform to generate 150-bp paired-end (PE) reads. .. Transcriptome libraries were constructed with a TruSeq RNA Library Prep Kit v2 (Illumina, CA, USA) with an insert size of 200–400 bp, after polyA selection, and sequenced on an Illumina NovaSeq 6000 platform, and 150-bp PE reads were generated. ..



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    Image Search Results


    Changes in intestinal short chain fatty acids and spleen transcriptome in mice treated with SYD. (A) The boxplot of acetic acid abundance among three groups; (B) The boxplot of isobutyric acid abundance among three groups; (C) Differences in PISA scores among the three groups; (D) Differences in body weight among three groups of mice; (E) and (F) The difference in thickness between the left and right ears of mice among the three groups; (G) The correlation between short chain fatty acids and phenotype indicators in mice, yellow represents positive correlation, blue represents negative correlation, and darker colors indicate higher correlation coefficients; (H) The volcano plot of transcriptome between Psoriasis and SYD Treatment groups; (I) The heatmap of inflammation related genes among significant differentially expressed genes; (J) The dot plot of GO enrichment analysis of upregulated genes; (K) The GSEA analysis of the G protein-coupled receptor signaling pathway; (L) The analysis of CIBERSORT, which displayed the relative abundance of different cell subpopulations in three groups; (M) The box plot shows significant differences in the abundance of plasma, Treg, and Tfh cell subpopulations among the three groups.

    Journal: Frontiers in Microbiology

    Article Title: Sanyin decoction alleviates psoriasis by reshaping gut microbiota and modulating the gut–spleen–skin axis

    doi: 10.3389/fmicb.2026.1799928

    Figure Lengend Snippet: Changes in intestinal short chain fatty acids and spleen transcriptome in mice treated with SYD. (A) The boxplot of acetic acid abundance among three groups; (B) The boxplot of isobutyric acid abundance among three groups; (C) Differences in PISA scores among the three groups; (D) Differences in body weight among three groups of mice; (E) and (F) The difference in thickness between the left and right ears of mice among the three groups; (G) The correlation between short chain fatty acids and phenotype indicators in mice, yellow represents positive correlation, blue represents negative correlation, and darker colors indicate higher correlation coefficients; (H) The volcano plot of transcriptome between Psoriasis and SYD Treatment groups; (I) The heatmap of inflammation related genes among significant differentially expressed genes; (J) The dot plot of GO enrichment analysis of upregulated genes; (K) The GSEA analysis of the G protein-coupled receptor signaling pathway; (L) The analysis of CIBERSORT, which displayed the relative abundance of different cell subpopulations in three groups; (M) The box plot shows significant differences in the abundance of plasma, Treg, and Tfh cell subpopulations among the three groups.

    Article Snippet: High-quality RNA was subsequently used for transcriptome library construction using the MGIEasy Fast RNA Reagent (MGI, 940-002921-00), mRNA was enriched using oligo (dT) magnetic beads, followed by fragmentation with fragmentation buffer at a controlled temperature.

    Techniques: Clinical Proteomics

    Changes in transcriptome of mouse skin after SYD treatment. (A) The volcano of differentially expressed genes; (B) The GO bioprocess (BP) analysis of significant upregulated genes; (C) The KEGG analysis of significant upregulated genes; (D) The GO molecular function (MF) analysis of significant upregulated genes; (E)-(H) The GSEA enrichment of significant regulated genes, NES < 0, represents downregulated signaling pathway, adjusted p value < 0.05, represents statistically significant; (I) The GSEA enrichment of upregulated signaling pathway; (J) The result of skin immune infiltration between Psoriasis group and Treatment group using CIBERSORT; (K) Boxplot of significant differences in immune cell subpopulations (Macrophage_M2, Neutrophils, Dendritic resting_cells).

    Journal: Frontiers in Microbiology

    Article Title: Sanyin decoction alleviates psoriasis by reshaping gut microbiota and modulating the gut–spleen–skin axis

    doi: 10.3389/fmicb.2026.1799928

    Figure Lengend Snippet: Changes in transcriptome of mouse skin after SYD treatment. (A) The volcano of differentially expressed genes; (B) The GO bioprocess (BP) analysis of significant upregulated genes; (C) The KEGG analysis of significant upregulated genes; (D) The GO molecular function (MF) analysis of significant upregulated genes; (E)-(H) The GSEA enrichment of significant regulated genes, NES < 0, represents downregulated signaling pathway, adjusted p value < 0.05, represents statistically significant; (I) The GSEA enrichment of upregulated signaling pathway; (J) The result of skin immune infiltration between Psoriasis group and Treatment group using CIBERSORT; (K) Boxplot of significant differences in immune cell subpopulations (Macrophage_M2, Neutrophils, Dendritic resting_cells).

    Article Snippet: High-quality RNA was subsequently used for transcriptome library construction using the MGIEasy Fast RNA Reagent (MGI, 940-002921-00), mRNA was enriched using oligo (dT) magnetic beads, followed by fragmentation with fragmentation buffer at a controlled temperature.

    Techniques:

    a Schematic illustration of the RNA-seq and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: A DNA-based nanodevice senses purinergic signaling and drives an immune switch for resolving inflammation

    doi: 10.1038/s41467-026-68879-2

    Figure Lengend Snippet: a Schematic illustration of the RNA-seq and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.

    Article Snippet: The RNA sequencing transcriptome library was generated with a TruSeq TM RNA sample preparation kit from Illumina (San Diego, CA). cDNA was synthesized via a SuperScript double-stranded cDNA synthesis kit (Invitrogen) with random hexamer primers (Illumina).

    Techniques: RNA Sequencing, Western Blot, Comparison