rna sequencing transcriptome library (Illumina Inc)
Structured Review

Rna Sequencing Transcriptome Library, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 12811 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transcriptome+libraries/TruSeq+RNA+Library+Prep/pmc12946169-402-1-16
Average 99 stars, based on 12811 article reviews
Images
1) Product Images from "A DNA-based nanodevice senses purinergic signaling and drives an immune switch for resolving inflammation"
Article Title: A DNA-based nanodevice senses purinergic signaling and drives an immune switch for resolving inflammation
Journal: Nature Communications
doi: 10.1038/s41467-026-68879-2
Figure Legend Snippet: a Schematic illustration of the RNA-seq and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.
Techniques Used: RNA Sequencing, Western Blot, Comparison
Related Articles
Construct:Article Title: To live or die: "Fine-tuning" adaptation revealed by systemic analyses in symbiotic bathymodiolin mussels from diverse deep-sea extreme ecosystems. Article Snippet: • High demand for activated gene regulation confirmed huge geochemical variety among ecosystems.. • Transition from active to extinct stage of cold seeps would be more severe than Article Title: Gene mapping and candidate gene analysis of a sorghum sheathed panicle‐I mutant Article Snippet: Three biological replicates were established for each developmental stage, and total RNA was extracted from the different tissues at each time point using TRIzol Reagent (Invitrogen). .. Article Title: Exploring potential targets and mechanisms of PCB52-induced cardiotoxicity: Integration of network toxicology and zebrafish experimental evidence. Article Snippet: RNA integrity was assessed using the RNA 6000 Nano Kit with the Agilent 2100 BioAnalyzer (Agilent Technologies, CA, USA). .. Subsequently, Article Title: Molecular insights into dye decolorization performance and mechanisms under carbon limited conditions in a membrane aeration-based bioelectrochemical system. Article Snippet: Bioelectrochemical systems (BES) is a promising strategy for azo dyes decolorization enhancement in carbonlimited condition, but decolorization products need further aerobic mineralization.. Here, a counter-diffusion biofilm-supported BES (E-MABR) was designed to achieve the decolorization, mineralization and denitrogenation of Alizarin Yellow R (AYR) in electron-deficient wastewater.. The introduction of electrodes facilitated the secretion of extracellular polymeric substances (EPS), particularly proteins (PN), whose content in the cathodic biofilm was 2.5 ± 0.2 times higher than that in the MABR. Article Title: Mechanistic analysis of the hardening process of the thorns on stems of Bougainvillea spectabilis Willdenow Article Snippet: .. Three biological replicates of samples in each stage were used to construct Article Title: Unravelling the postharvest quality diversities of different sweet corn varieties Article Snippet: Different varieties of sweet corn exhibit varying degrees of quality deterioration after harvest, especially changes in appearance, sugar composition, texture and flavor.. Changes of 18 common phenolic acids, organic acids, and sugars of sweet corn varieties, ‘Jingkenuo 2000’, ‘Jingkenuo 768’ and ‘Nongkenuo 336’, during a shelf-life period of 10 d at 20 C were identified in this study.. The differences of flavor among the varieties may be due to their different flavor regulation pathways. Article Title: Chromosome-level genome assembly of Niphotrichum japonicum provides new insights into heat stress responses in mosses Article Snippet: DNA libraries for short-read whole genome sequencing (WGS) were constructed using the Illumina TruSeq DNA PCR-free library preparation kit (Illumina, CA, USA) with 300- to 500-bp fragment sizes, and sequenced on the Illumina NovaSeq 6000 platform to generate 150-bp paired-end (PE) reads. .. Article Title: Unveiling detoxifying symbiosis and dietary influence on the Southern green shield bug microbiota Article Snippet: Prior to sequencing, bacterial mRNA was purified with the MICROBExpress Bacterial mRNA Enrichment Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Next-Generation Sequencing:Article Title: Gene mapping and candidate gene analysis of a sorghum sheathed panicle‐I mutant Article Snippet: Three biological replicates were established for each developmental stage, and total RNA was extracted from the different tissues at each time point using TRIzol Reagent (Invitrogen). .. Sample Prep:Article Title: Molecular insights into dye decolorization performance and mechanisms under carbon limited conditions in a membrane aeration-based bioelectrochemical system. Article Snippet: Bioelectrochemical systems (BES) is a promising strategy for azo dyes decolorization enhancement in carbonlimited condition, but decolorization products need further aerobic mineralization.. Here, a counter-diffusion biofilm-supported BES (E-MABR) was designed to achieve the decolorization, mineralization and denitrogenation of Alizarin Yellow R (AYR) in electron-deficient wastewater.. The introduction of electrodes facilitated the secretion of extracellular polymeric substances (EPS), particularly proteins (PN), whose content in the cathodic biofilm was 2.5 ± 0.2 times higher than that in the MABR. Article Title: Unravelling the postharvest quality diversities of different sweet corn varieties Article Snippet: Different varieties of sweet corn exhibit varying degrees of quality deterioration after harvest, especially changes in appearance, sugar composition, texture and flavor.. Changes of 18 common phenolic acids, organic acids, and sugars of sweet corn varieties, ‘Jingkenuo 2000’, ‘Jingkenuo 768’ and ‘Nongkenuo 336’, during a shelf-life period of 10 d at 20 C were identified in this study.. The differences of flavor among the varieties may be due to their different flavor regulation pathways. RNA Extraction:Article Title: Unravelling the postharvest quality diversities of different sweet corn varieties Article Snippet: Different varieties of sweet corn exhibit varying degrees of quality deterioration after harvest, especially changes in appearance, sugar composition, texture and flavor.. Changes of 18 common phenolic acids, organic acids, and sugars of sweet corn varieties, ‘Jingkenuo 2000’, ‘Jingkenuo 768’ and ‘Nongkenuo 336’, during a shelf-life period of 10 d at 20 C were identified in this study.. The differences of flavor among the varieties may be due to their different flavor regulation pathways. Selection:Article Title: Chromosome-level genome assembly of Niphotrichum japonicum provides new insights into heat stress responses in mosses Article Snippet: DNA libraries for short-read whole genome sequencing (WGS) were constructed using the Illumina TruSeq DNA PCR-free library preparation kit (Illumina, CA, USA) with 300- to 500-bp fragment sizes, and sequenced on the Illumina NovaSeq 6000 platform to generate 150-bp paired-end (PE) reads. .. Generated:Article Title: Chromosome-level genome assembly of Niphotrichum japonicum provides new insights into heat stress responses in mosses Article Snippet: DNA libraries for short-read whole genome sequencing (WGS) were constructed using the Illumina TruSeq DNA PCR-free library preparation kit (Illumina, CA, USA) with 300- to 500-bp fragment sizes, and sequenced on the Illumina NovaSeq 6000 platform to generate 150-bp paired-end (PE) reads. .. |
